Signal regulatory protein Alpha (SIRPAlpha), a transmembrane protein that is predominantly expressed in dendritic cells (DCs) or\nmacrophages, interacts with CD47 that is overexpressed in almost all types of tumor cells. The interaction between SIRPAlpha and\nCD47 leads to a negative signal that prevents the phenotypic and functional maturation of DC and inhibits phagocytosis. The\nSIRPAlpha knockdown in DCs that were pulsed with a modified HPV16E7 (HPV16mE7) protein with enhanced antigenicity and\nreduced transformation activity results in increased cytokine (TNF-Alpha/IL-12/IL-6) secretion, IFN-Gamma secretion by T lymphocytes,\nand in vitro/in vivo tumoricidal activity against cervical cancer cells. Taken together, these results suggest that SIRPAlpha-silenced\nDC vaccination presented potential therapeutic implications against cervical cancer.
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